Gut microbiota: next frontier in understanding human health and development of biotherapeutics

The gut microbiota is a remarkable asset for human health. As a key element in the development and prevention of specific diseases, its study has yielded a new field of promising biotherapeutics. This review provides comprehensive and updated knowledge of the human gut microbiota, its implications in health and disease, and the potentials and limitations of its modification by currently available biotherapeutics to treat, prevent and/or restore human health, and future directions. Homeostasis of the gut microbiota maintains various functions which are vital to the maintenance of human health. Disruption of the intestinal ecosystem equilibrium (gut dysbiosis) is associated with a plethora of human diseases, including autoimmune and allergic diseases, colorectal cancer, metabolic diseases, and bacterial infections. Relevant underlying mechanisms by which specific intestinal bacteria populations might trigger the development of disease in susceptible hosts are being explored across the globe. Beneficial modulation of the gut microbiota using biotherapeutics, such as prebiotics, probiotics, and antibiotics, may favor health-promoting populations of bacteria and can be exploited in development of biotherapeutics. Other technologies, such as development of human gut models, bacterial screening, and delivery formulations eg, microencapsulated probiotics, may contribute significantly in the near future. Therefore, the human gut microbiota is a legitimate therapeutic target to treat and/or prevent various diseases. Development of a clear understanding of the technologies needed to exploit the gut microbiota is urgently required

Microbial Biotransformation of a Polyphenol-Rich Potato Extract Affects Antioxidant Capacity in a Simulated Gastrointestinal Model

A multistage human gastrointestinal model was used to digest a polyphenol-rich potato extract containing chlorogenic acid, caffeic acid, ferulic acid, and rutin as the primary polyphenols, to assess for their microbial biotransformation and to measure changes in antioxidant capacity in up to 24 h of digestion. The biotransformation of polyphenols was assessed by liquid chromatography–mass spectrometry. Antioxidant capacity was measured by the ferric reducing antioxidant power (FRAP) assay. Among the colonic reactors, parent (poly)phenols were detected in the ascending (AC), but not the transverse (TC) or descending (DC) colons. The most abundant microbial phenolic metabolites in all colonic reactors included derivatives of propionic acid, acetic acid, and benzoic acid. As compared to the baseline, an earlier increase in antioxidant capacity (T = 8 h) was seen in the stomach and small intestine vessels as compared to the AC (T = 16 h) and TC and DC (T = 24 h). The increase in antioxidant capacity observed in the DC and TC can be linked to the accumulation of microbial smaller-molecular-weight phenolic catabolites, as the parent polyphenolics had completely degraded in those vessels. The colonic microbial digestion of potato-based polyphenols could lead to improved colonic health, as this generates phenolic metabolites with significant antioxidant potential

Dates and rates of endo-exorheic drainage development : insights from fluvial terraces (Duero River, Iberian Peninsula)

Fluvial terraces are valuable records to study and characterize landscape evolution and river response to base level lowering, and to decipher coupled responses between fluvial incision and regional tectonics. The opening of closed basins has a strong impact on fluvial dynamics, as it involves an abrupt base level lowering that accelerates landscape fluvial dissection. This study focuses on the time response of the Duero Basin, the largest and best preserved among the Cenozoic basins of the Iberian Peninsula, to exorheism. Fluvial incision due to basin opening has developed up to 13 un-paired strath terraces along the south margin of the Duero river, distributed at relative heights up to + 136-128 m compared to the modern floodplain. Paired Be-10-Al-26 cosmogenic isotope depth profiles from six fluvial terraces, located ca. 30-80 km upstream from the opening zone, suggest Pleistocene ages for almost the entire fluvial terrace staircase (from T3 at +112 -107 m, to T12 at + 13-11 m). The terrace density and the total lowering of the terrace surface, key parameters in limiting terrace exposure ages, were estimated based on field and geomorphological data. Apparent burial durations and basin denudation rates deduced from inherited Be-10-Al-26 concentrations provide valuable information on basin evolution. Apparent basin denudation rates remained relatively low (< 3-6 m.Ma(-1)) during the Pliocene, and doubled (8-13 m.Ma(-1)) during the Early Pleistocene (ca. 2-1 Ma) possibly showing a lower proportion of recycled sediments. Time averaged incision rates deduced from terraces in the study area and along some tributaries show that incision rates are higher close to the opening site (122- < 250 m.Ma(-1)) than towards the upstream part of the catchment (88-68 m.Ma(-1)), evidencing the retrogressive travel of the erosive wave nucleated at the opening site